Changelog

Last Update 8/6/24

• Improved cell cycle and kinetics user interface
• Legend can be moved to the side of the plot
• Plate Map (beta)
• Display gates if x/y axis flipped
• Display poly gates if able to when axis scale changes
• Bug fixes

8/6/24

• Improved cell cycle and kinetics user interface
• Legend can be moved to the side of the plot
• Plate Map (beta)
• Display gates if x/y axis flipped
• Display poly gates if able to when axis scale changes

6/26/24

• Export FlowJo¹ workspace (.wsp) file to open your analysis in FlowJo.
• Improved support for importing FlowJo¹ workspace files
• Import/export Archival Cytometry Standard (ACS) files
• Import Zipped FCS Files
• GatingML files are now imported as a pipeline. Easy to drag files to the pipeline group to apply all gates
• Rect and Quad gates are now visible on plot regardless of whether the current plot transforms match the gate transforms
• New Autospill Compensation Settings - enable/disable automatic updating of matrix when you change a bounding gate
• Improved pipeline color selection - populations retain the same color through out the pipeline

5/5/24

• Custom Names for Autospill Parameters
• Parameter Spectrum Plots

3/3/24

• Import CSV Files
• Added Geometric Mean stat

2/21/24

• Pipeline filters
• Improved support for loading FlowJo¹ workspaces with Groups
• Improved error reporting when loading workspaces with missing fcs files

2/12/24

• SVG Image Exports
• Pipeline Templates
• Pipeline transforms
• Pipeline CSV Exports

1/28/24

• Pipeline tools

1/14/24

• Mulitple Pipelines
• Stacked and Spread Histogram Overlays
• Improved UI Dialogs

11/25/23

• FCS3.2 support

11/5/23

• Pipeline Gate Editing

10/28/23

• Downsampling

10/8/23

• Multiple plots
• Color Gates

8/12/23

• Basic support for tSNE

6/27/23

• Added more FlowJo¹ workspace support.

5/14/23

• Started adding support for importing FlowJo¹ workspaces.
• Added a setting for File Name Pattern

3/13/23

• New Design
• Workspaces save more things

2/12/23

• Added basic URL loading
• Minor bug fixes

12/4/22

• Several Bug Fixes

9/29/22

• Added Kinetics tool

7/25/22

• Pipelines - Quickly and easily apply a gating and statistic hierarchy to all your files.
• Batch image exporting of pipelines
• Improvements to Cell Cycle engine, including better curve fitting and more statistics
• Offscreen and undefined event warnings

5/10/22

• Major Update
• Complete rewrite of all computational expensive code in WebAssembly

Additions/Improvements

• Significant performance improvements, especially file loading, gating, and rendering
• A new legend editor where you can not only select the color of overlays but rename and change the order of legend entries as well
• Exporting events for a specific gate, not just files
• Better File Tree with additional features such as Copy/Paste
• Improvements to CustomParameter dialog
• Overall more polished UI

Changes - Some are Breaking Changes

• The new version requires modern web browser features, so older browsers are not supported
• The recent versions of Google Chrome, MS Edge, and Firefox will all be fine. The latest Safari (version 15.2) should work but will be several times slower than the rest
• Read more about browser support here
• The saved workspace file format is different, so old saved workspaces won't work completely
• Events are no longer exported to CSV files in the "File" menu. They are exported by right clicking the population in the file tree and selecting "Export Events"
• Polygon gates are drawn by connecting the first and last points, not with a double click, which was hard to use
• Plot overlays can only be created by dragging and dropping files from the file tree onto the plot
• Boolean gates are now created by selecting gates in the file tree, right clicking, and selecting the desired boolean type
• Gates are not linked when you copy them (changing a gate doesn't change all of its copies). We are working on a better way to do this in a future release
• We have temporarily removed batch image saving. This will come back in the next release

12/10/21

• Mobile and tablet bug fixes
• Performance improvements for compensation

11/21/21

• Upgraded our infrastructure for improved page load times
• New fcs file loading system which is up to 10x faster

6/3/21

• Better Workspace saving including statistics, custom parameters, boolean gates, and more
• Better (near complete) GatingML 2.0 support - ratio transforms, bounded transforms, 3D+ gates
• Drag and drop external files onto webpage to open them
• Much more powerful Custom Parameters - with multiple parameters, constants, builtin functions
• Custom manually-input gates (right-click on plot)
• Graphics performance enhancements and a few minor bug fixes

5/6/21

• Started basic persistant settings
• Can turn off gate linking entirely
• Added support for a few weird cytometers
• A couple bug fixes

5/3/21

• Export JPEGs
• Faster page loading
• Better axis scaling selector

4/27/21

• Full multi-select on population panel using ctrl/cmd
• Dot plot overlays
• Drag and drop populations onto plot/histogram to easily create overlays
• Can select custom colors for overlays (right-click on plot)
• New histogram display options - line thickness, fill color (right-click on plot)
• Ability to export the events in a file as CSV

4/14/21

• New image exporting menu with a bunch of new options, including batch processing
• Custom parameter support for creating parameters derived from others

4/2/21

• Made stats renamable
• Made files renamable
• Option to unlink all gates in a file
• Can manually set histogram absolute maximum
• Can hide histogram legend
• Allow histogram overlaying of any file with compatible parameters
• Able to select (with CTRL/CMD) and drag multiple gates from the same file

3/24/21

• Normalized Time parameters to seconds
• Added Min/Max stats
• Added gate details to see bounds of gates
• Added ability to move gate labels around the plot
• Added ability to change plot point size and gate line thickness

3/16/21

• Added ability to unlink gates
• Added support for some FCS2.0 files
• Better workspace saving
• Bug fixes

2/23/21

• Added Automatic Compensation - AutoSpill
• Added new histogram axis scales
• Improved graphics
• Bug fixes

01/28/21

• Async compensation
• Fixed bug with bitmask for FCS files with 32 bit range
• Better axis selection when you drop a gate
• Better cycling
• Improved axis drawing

01/19/21

• Progressive Web App support
• New Analysis page design
• Experimental Cell Cycle analysis support

01/06/21

• New website design
• Added Boolean Gates
• Added Hyperlog Transform

12/2/2020

• Added DBScan clustering

11/9/2020

• Added KMeans Clustering

10/19/2020

• Added gate exporting/importing with XML format

9/2/2020

• Added Logicle Transform

[1] - We have no affliation with FlowJo or its parent company