When a new file is opened for the first time a dot plot is displayed for it. By default Floreada tries to display FCS/SSC on X/Y axis if possible. Parameters in FCS files are given two names, a short name and a long name. These names are stored in two keywords $PnN and $PnS, where "n" is the parameter number. Floreada will display the full parameter name as "Long Name" :: "Short Name" unless the two are the same and then it will just display it once. Currently all dot plots are displayed in color representing the event density with blue being least dense, cyan, then green, then yellow, and finally red being most dense.
In order to change parameters, click on the current x or y parameter name. A popup menu will display with all the currently available parameter names, where you can select the new parameter for the axis.
To change the current axis, click on the axis and a popup will display. A histogram is shown to help you visualize the distribution of events as you change the scale. You can adjust the minimum and maximum axis values as well as switch between the following scale types:
Logicle is a special transformation developed at Stanford. It puts events near zero on a linear scale and bigger events on a log scale. It helps to spread out big groups of events around zero, which can be a problem if you visualize data on a logarithmic scale. Another plus of Logicle is that unlike regular Logarithmic scaling, it is defined for negative events. Some FCS files do contain negative data and events may become negative if compensated as well. If these are displayed on a regular log scale, they will be ignored but Logicle can evaluate and display the negative events.
There are several parameters that you can set when specifying a new Locigle transform:
- Max (or T) - The top of scale value
- W - The number of decades in the linear region
- M - The number of decades in the log region
- A - The number of additional decades for negative data values
Floreada will fill in reasonable settings to start with but you can change any value you want to.
Changing Point Size
The size of the points of a plot can be adjusted. Right click on the plot and under the "Point Size" menu option, you can select from several possible sizes.
Download Plot Image
If you would like to save the current plot image, click "File" then "Save Image". This will open a preview window where you can select from a bunch of different options, and can enable or disable multiple different aspects of the current plot from appearing in the image, such as the plot title, the axis label, etc. You can also customize the displayed X and Y axis labels if needed.
Currently, images are saved as PNG or JPEG files but we can add more file types if needed.