Overlays allow you to view two or more different populations in the same plot. Overlays can be applied to both dot plots and histograms.
Overlays can be created in two ways. The easiest way is by selecting one or more populations from the side panel and then drag-and-dropping them onto the plot. Alternatively, you can click the overlay button in the lower-left corner of the plot and select all populations that you would like to apply as overlays.
When overlays are applied, a few things happen. First a legend appears in the upper-left corner of the plot. Additionally in the population tree, overlays are highlighted with their corresponding overlay color.
To be able to overlay a file, it must have all of the exact same parameters in the same order as the main file, not just the parameters that are displayed.
All overlays can be removed from a plot by right-clicking on the plot and selecting "Clear Overlays". If you would only like to remove a particular overlay, you can do that be either right-clicking the population in the population tree and then selecting "Remove Overlay", or by clicking the Overlay Button in the lower-left corner of the plot and deselecting the population.
There are several options to change the way that overlays are displays. Options can be accessed by right-clicking on the plot. Display Options include:
The legend can be toggled on or off by right-clicking the plot and selecting "Hide Legend."
You can change the color assigned to a particular overlay by right clicking the plot and selecting "Colors". A popup menu will appear which will allow you to select any color that you want for each overlay.