Currently, there are six available gates: Rectange, Polygon, Range, Quadrant, Ellipse, and Boolean
To create a new gate for the current file, select a gate type from the gating panel. The method to draw a gate differs depending on the type of gate.
Click on the plot to place a vertex of the rectangle. Then drag to draw the rectangle and release to finalize the gate.
Click to place vertices of the polygon. Double click to finalize the gate.
Click on the plot to place a bound of the range. Then drag to draw the range and release to finalize the gate.
Click on the plot to place the center of the quadrants. Release to finalize the gate.
Click on the plot to place one end of the ellipse. Then drag to draw the ellipse and release to finalize the gate.
Boolean gate are create differently. After selecting the Boolean gate tool, a popup menu will allow you to select existing gates at the same population level and a boolean operation to create a new gate.
With the exception of quadrant gates and boolean gates, when a gate is finalized, a popup will appear allowing you to name the new gate.
To create a sub-gate, select a gate to apply it to a file and then draw the new child gate.
Once a gate is created, the stats for the gate will be calculated and displayed. If the gate is a top-level gate, the percentage shown will be the percent of total events that are contained in the gate. If the gate is a sub-gate, two percentages will be shown. The first percentage is percent of total events contained in the gate. The second percentage is the percent of events in the parent gate that are contained in the child gate.
To apply a gate and mask out all events not contained within it, click the gate name from the file/gate tree.
In order to delete a gate, right click on the gate and click "Delete". The gate and all sub-gates will be removed. This can not be undone.
To copy a gate from one population and apply it to a different population, you can drag the gate from the population tree and drop it onto either another population. To do this, the target population must have parameters that match the original gate parameters exactly. There is currently no way to manually reconcile parameter names that differ between files.
You can select multiple gates to drag-and drop at the same time by holding ctrl/cmd.
If you want to apply the gates in one file to all the files in a project, select the file you want to copy from and press "Control + A".
When a gate is copied to another file, those gates become linked. Changes to the gate in any file will update the gate in all the files that use it.
All gates can be edited after they are created. To edit a gate, click on it in the plot. Once it is selected, its vertices will be highlighted. From there, you can drag the gate as a whole to a new position or drag individual to adjust its size and shape. Again, if a gate has been applied to multiple files, all instances of the gate will change when it is edited and statistics will be updated for all files.
Once a gate is selected in the plot, you can also drag-and-drop the gate label to anywhere on the plot.
By default, when you drag a gate from one population to another, the two gates are linked. This means that if you edit the gate in one place, it will be changed for all instances of the gate. If you select a gate in the population tree, all gates that are linked to it will be highlight with italics. If you would like to prefer to unlink gates so that editing a gate does not apply the change globally you can right click the gate in the population tree and select "Unlink". Note that if you take a gate which you have unlinked and drag-and-drop it again, it will be linked again to the new gate.
To view the details of a gate, right-click it in the population tree and select "Details". The details popup menu will will show you the exact bounds of a gate which may be useful in certain situations.
In addition to seeing the exact bounds of a gate in the details menu, you can also rename the gate if you need to.
The appearence of gates on the plot can be altered by right-clicking the plot and changing the following display options:
The thickness of the gate lines can be set to 'thin', 'medium', or 'thick'.